Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: A stereological examination

Sherkatolabbasieh, Hamidreza and Hagh-Nazari, Lida and Shafiezadeh, Shiva and Goodarzi, Nader and Mehdi Zangeneh, Mohammad and Zangeneh, Akram (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: A stereological examination. ARCHIVES OF BIOLOGICAL SCIENCES, 69. pp. 535-543. ISSN 0354-4664

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Abstract

Abstract: The present study was carried out to investigate the nephroprotective effect of the ethanolic extract of Allium saralicum R.M. Fritsch (ASRMF) in mice. Thirty-five male mice were divided into five groups (n=7). Group 1 (positive control) received 1 mL/kg olive oil intraperitoneally (i.p.) and 0.5 mL distilled water orally; Group 2 (negative control) received CCl4 (50% in olive oil, 1 mg/kg; i.p.); Groups 3, 4 and 5 received CCl4 and 200, 800 and 1600 μg/kg of ASRMF extract, respectively. The renal volume and cortex in Groups 1 and 2 were increased by 55% and 62% (p≤0.001) following CCl4 administration, respectively, and were improved after ASRMF administration. The volume of proximal convoluted tubules (PCTs), glomeruli, vessels and interstitial tissue increased 80%, 150%, 83% and 64% (p≤0.05), respectively, in CCl4- treated mice, and decreased significantly with 800 and 1600 μg/kg of ASRMF. The length of PCTs and vessels increased 51% and 45% and decreased (p≤0.05) with 200, 800 and 1600 μg/kg of ASRMF, respectively. CCl4-treated mice lost 22.5% of glomeruli; the loss was inhibited significantly (p≤0.05) by ASRMF. Urea and creatinine concentrations were increased (p≤0.05) in CCl4-induced nephrotoxicity as compared to the controls, whereas different doses of ASRMF restored the levels of these biomarkers compared to the negative controls. In conclusion, ASRMF has a potent nephroprotective property and can improve renal structural and serum biomarkers in CCl4-induced nephrotoxicity in mice. Key words: carbon tetrachloride; Allium saralicum; kidney; nephrotoxicity; stereology

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