The study and identification of Quorum Sensing (QS)‎‏ ‏genes of Pseudomonas ‎aeruginosa strains isolated from human clinical samples by Multiplex ‎PCR and antibiotic resistance determination

Amini Bezanjanii, Firozeh and Mahmoudi*, Razegh and Amini, Kiuomars (2016) The study and identification of Quorum Sensing (QS)‎‏ ‏genes of Pseudomonas ‎aeruginosa strains isolated from human clinical samples by Multiplex ‎PCR and antibiotic resistance determination. yafte, 18 (2).

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Abstract

Background : Pseudomonas aeruginosa is an opportunistic pathogen and the cause of 10% to 15% of ‎nosocomial infections.Virulence genes of Pseudomonas aeruginosa is one of the most ‎aggressive mechanisms and the issue of medical opinion is important. The expression of many ‎genes is controlled and regulated in pathogenic bacteria Pseudomonas aeruginosa gene by a ‎system called Quorum Sensing (QS). QS controls and regulates cell-to-cell communication ‎system using small molecules in single-celled organisms SMs. This study was aimed at ‎defining the prevalence of QS genes in Pseudomonas aeruginosa isolated from human ‎resources.‎ Materials and Methods: Initially 60 samples of Pseudomonas aeruginosa from human intestinal samples were prepared ‎and confirmed by culture-specific diagnostic tests. Multiplex PCR test was ‎performed to detect genes. Antimicrobial susceptibility of the isolates against 10 antimicrobial agents was ‎determined using standard disk diffusion method.‎ Results: Multiplex PCR results showed that the frequency of the desired genes rhlR 5 %, lasR 48.3% and lasI 60%. The genes lasB, apr, rhlAB and rhlI were not detected in any of the samples. According to antibiogram, the most resistance was against amikacin, amoxicillin and cefotaxime and the most susceptibility was to ciprofloxacin and ceftazidime. Conclusion: This study shows high prevalence of Quorom Sensing genes in Pseudomonas aeruginosa isolates of human origin. Keywords: Pseudomonas aeruginosa, Quorum Sensing.

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