Mandian, Somayeh) and Sadeghifard, Nourkhoda and Pakzad, Raj and (Ghanbari,, Fatemeh and Soroush, Setareh and Azimi, Lila) and Rastegar-Lari, Abdolaziz and Giannouli,, Maria and Taherikalani,, Morovat) (2015) Acinetobacter baumannii clonal lineages I and II harboring different carbapenem-hydrolyzing-beta-lactamase genes are widespread among hospitalized burn patients in Tehran. JOURNAL OF INFECTION AND PUBLIC HEALTH, 8 (6). pp. 533-542. ISSN 1876-0341
Full text not available from this repository.Abstract
The aim of this study was to analyze antimicrobial resistance patterns and their encoding genes and genotypic diversity of Acinetobacter baumannii isolated from burn patients in Tehran, Iran. The presence of extended-spectrum beta-lactamase- and bla(OXA)-encoding genes among 37 multidrug resistant (MDR) A. baumannii strains isolated from patients hospitalized in a teaching hospital in Tehran was evaluated. Susceptibility to 7 antibiotics was tested by disk agar diffusion and to polymyxin B and colistin was tested by E-test, according to CLSI guidelines. All isolates were then analyzed by PCR for the presence of bla(IMP), bla(VIM), bla(SIM) bla(OXA-23), bla(OXA-24), and bla(OXA-58)-like carbapenemase genes, and bla(OXA-51)-like, bla(TEM), bla(SHV), bla(PER), bla(VEB), and bla(GIM) genes. Genotyping of A. baumannii strains was performed by repetitive sequence-based (REP)-PCR and cluster analysis of REP-PCR profiles. A. baumannii isolates were assigned to international clones by multiplex PCR sequence group analysis. Twenty-five A. baumannii isolates were classified as MDR, and 12 were classified as extensively drug resistant. All isolates were susceptible to colistin and polymyxin B. Eighty-one percent of the isolates was resistant to imipenem or meropenem and harbored at least one or both of the bla(OXA-23)-like or bla(OXA-24)-like carbapenemase genes. Co-existence of different resistance genes was found among carbapenem-resistant isolates. Multiplex PCR sequence group analysis most commonly assigned A. baumannii isolates to international clones 1(18/37; 48.6%) and 11(18/37; 48.6%). An alarming increase in resistance to carbapenenns and the spread of bla(OXA-23)-like and/or bla(OXA-24)-like carbapenennase genes was observed among A. baumannii strains belonging to clonal lineages I and II, isolated from burn patients in Tehran. 0 2015 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Limited. All rights reserved.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > SB Plant culture |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 23 Oct 2017 12:04 |
| Last Modified: | 23 Oct 2017 12:04 |
| URI: | http://eprints.lums.ac.ir/id/eprint/682 |
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