Mohammadzadeh, Adel and Pourfathollah, Ali Akbar and Shahrokhi, Somayeh and Fallah, ali and Tahoori, Mohammad Taher and Amari,, Afshin and Forouzandeh, Mahdi and Soleimani, Masoud (2016) Evaluation of AD-MSC (Adipose derived –Mesenchymal stem cells) as a vehicle for IFN-β delivery in Experimental Autoimmune Encephalomyelitis. Clinical Immunology.
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Abstract
Interferon-β (IFN-β) is commonly used as a disease modifying drug for the treatment of Relapseremitting multiple sclerosis (RR-MS). However, the underlying mechanism by which IFN-β mediate this immunosuppressive effect is still unknown. In this study, we analyzed the effects of genetically modified Adipose Derived Mesenchymal Stem Cells (AD-MSCs) expressing murine interferon beta (MSCs-VP/IFN-β) on the animal model of MS, experimental autoimmune encephalomyelitis (EAE). Lymph node mononuclear cells and serum were examined by using RT-PCR and ELISA methods to measure the production of IL-10 and IL-17 gene and protein expression, respectively. Our results indicated that in the MSCs-VP/IFN-β treated group induction of Tregs and IL-10 and reduction of IL-17 were significant. Taken together, we showed that using AD-MSCs expressing IFN-β as an anti-inflammatory agent, offer evidence supporting that the stem cell therapies in EAE conceivably will improve the valuable effects of IFN-β in this autoimmune disease. Key words; MSCs (Mesenchymal Stem Cells), IFN-β, Treg, EAE
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 08 May 2017 05:08 |
| Last Modified: | 18 Nov 2017 15:37 |
| URI: | http://eprints.lums.ac.ir/id/eprint/568 |
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