Phospholipase and proteinase activities of Candida spp. isolates from vulvovaginitis in Iran

Shirkhani, S and Sepahvand, A and Mirzaee, M and Anbari, K (2016) Phospholipase and proteinase activities of Candida spp. isolates from vulvovaginitis in Iran. Journal de Mycologie Médicale. pp. 255-260.

Preview

Text shirkhani2016.pdf Download (375kB) | Preview

Abstract

Summary Objective of the study. — This study aims to characterize phospholipase and proteinase activities of Candida isolates from 82 vulvovaginal candidiasis (VVC) and to study the relationship of these activities with vulvovaginitis. Methods. — Totally 82 Candida isolates from vagina samples of VVC patients were randomly collected over the period between September and December 2014 from hospitalized patients at the general hospitals of Lorestan province, Iran. Isolates were previously identified by conventional mycological methods. The phospholipase and proteinase activities were evaluated by Egg yolk agar, Tween 80 opacity medium and agar plate methods. Results. — The most common Candida species was identified Candida albicans (n = 34, 41.5%), followed by Candida famata (n = 13, 15.8%), Candida tropicalis (n = 11, 13.4%), and Candida parapsilosis (n = 9, 11%). The most phospholipase activity was observed in Candida colliculosa (40%), followed by C. famata (38.5%), and Candida krusei (33.3%). The findings revealed that the correlation between phospholipase production by Candida spp. and the presence of VVC was not found to be statistically significant (P = 0.91). All Candida spp. exhibited considerable proteinase activity; so that 100% of C. colliculosa, C. parapsilosis, Candida kefyr, and Candida intermedia

Actions (login required)

View Item