DIAGNOSIS OF NON-POLIO ENTEROVIRAL INFECTIONS IN SEPTIC NEONATES BY POLYMERASE CHAIN REACTION ASSAYS

Razlansari, Ali Akbar and Jamalidoust, Marzieh and Ziyaeyan, Mazyar and Shahian, Mozhgan and Moeini, Mahsa and Asaei, Sadaf and Obeidavi, Zia and Alborzi, Abdolvahab (2016) DIAGNOSIS OF NON-POLIO ENTEROVIRAL INFECTIONS IN SEPTIC NEONATES BY POLYMERASE CHAIN REACTION ASSAYS. IJPSR, 7.

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Abstract

ABSTRACT: Objective: The purpose of this study was to investigate the prevalence of non-polio enteroviral infections by quantitative and qualitative PCR assays in neonates 3-30 days old with clinical diagnosis of sepsis. Methods: The patients included all the neonates aged 3-30 days admitted with diagnosis of neonatal sepsis. Blood specimens, throat swabs, and in 60 cases CSF specimens were tested for Enteroviruses (EVs), using a quantitative RT-PCR assay. Alternate RT-PCR assays separated polio and non-polio enteroviruses. Demographic data including age, sex, type of feeding and gestational age were obtained. Results: As PCR results revealed, of the 177 neonates with clinical diagnosis of sepsis, one patient (0.55%) was positive for non-polio EVs in all of throat, serum and CSF samples. In 3 patients (1.7%), the results were positive for non-polio EVs in both throat and serum, while in15 (8.45%) others only throat samples were positive. Sex, age and gestational age had no significant relationship with the prevalence of the infections statistically (P>0.05). The prevalence of non-polio EV infections ranged from 6.1% in spring to 15.4% in fall with a significantly increased rate in patients with ill contacts within the family or others (P= 0.05) and inversely related with birth weight (P=0.02). Conclusion: Non-polio EV infections are an important cause of sepsis –like illnesses in neonates. Real-time PCR can serve as a rapid and specific method for the diagnosis of non-polio EVs in neonates suspected to sepsis

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