The effect of quercetin on serum antioxidant properties of hind limb ischemia-reperfusion injury in adult rat

Gholami *, Mohamad reza and Ahmadvand, Hasan and Anbari, KHatre and Khanipour-khayat, Zahra and Hadipour, Forozan (2015) The effect of quercetin on serum antioxidant properties of hind limb ischemia-reperfusion injury in adult rat. yafte, 17 (3).

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Abstract

Background : Quercetin is a member of flavonoids family which can be found in vegetables, fruits, tea and dietary supplements. Quercetin has anti-inflammatory, antibacterial and antioxidant properties. This study evaluated the effect of quercetin on serum antioxidant properties of hind limb ischemia-reperfusion injury in adult rat. Materials and Methods : 72 adult male Wistar rats weighing 250 -300 g were used. They were divided into 9 groups (N=8). The femoral artery and vein was occluded using silk 6.0 with slip knot technique. All groups underwent ischemia for 3 hours and reperfusion was applied 3 days, 7 days, 14 days, 28 days. In half of each the experimental group150 mg/kg quercetins was injected intraperitoneally after ischemia. Blood samples were collected before killing the rats to measure the level of enzyme activity GPX, CAT, NO, POX and MDA. Results: Serum enzyme activities of GPX, CAT, NO, POX, MDA in group received quercetin compared with the control group showed that quercetin reduces the level of serum activity of NO and POX. Conclusion: Quercetin can ameliorate damage caused by free radicals in the process of hind limbs ischemia-reperfusion via decrease NO activity levels. Keywords: Reperfusion, Hind limb, Quercetin, Antioxidant

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