Combination of zinc nanoparticles with chitosan scaffolds increased cytokine genes on wound healing of infected rats with methicillin-resistant Staphylococcus aureus (MRSA)

Shahmoradi, Mohammad Kazem and Amini Nogorani, Mehrdad and Mansouri, Fatemeh and Zarei, Leila (2023) Combination of zinc nanoparticles with chitosan scaffolds increased cytokine genes on wound healing of infected rats with methicillin-resistant Staphylococcus aureus (MRSA). Advancements in Life Sciences.

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Abstract

Background: The present study aims to determine the effect of zinc nanoparticles with chitosan in the expression of cytokine genes on wound healing of infected rats with methicillin-resistant Staphylococcus aureus (MRSA). Methods: In this study, all male Wistar rats were divided into five groups. Group M1: 0.1 mL sterile saline 0.9% solution was added to the wounds with no infection. Group M2: the wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA) and only treated with 0.1 mL the sterile saline 0.9% solution. Group M3: animals with infected wounds were treated with zinc nanoparticle. Group M4: animals with infected wounds were treated with chitosan. Group M5: animals with infected wounds were treated with 0.1 mL solution of zinc nanoparticles with chitosan. Skin biopsy samples were removed for the histological studies and quantitative assessment of IL-6, VEGF, TNF and TGF genes using real-time PCR in each groups. Results: Quantitative histological and neovascularization studies showed that there was significant difference between rats in groupM5 compared to other groups. The infected groupM5 exhibited a significant increase in the expression levels of VEGF: 8.02, TNF: 5.34, TGF: 7.98, and decrease of IL-6:-3.34 folds as compared to the other groups on the 21st day (P<0.05). Also, on the same day was seen the minimum surface area of wound in group 5. The surface area between each study group and other groups was statistically significant(P<0.05). Conclusion: Our studies also show that the type of zinc nanoparticles with chitosan scaffolds have more effects than other types of compounds in wound healing.

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