Mirzadeh, Sedigheh and Mahmoudi*, Razagh and Amini, Kiumars (2016) Evaluation of virulence genes in the Salmonella enteritidis isolated from food samples by multiplex-PCR. Yafte, 18 (3).
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Abstract
Background : Salmonella enterica serotype Enteritidis is one of the most important cause of non-typhoidal salmonellosis, a clinically less severe infection than typhoid fever. The present study aimed to detection of virulence genes in the S. enteritidis isolated from the food samples by multiplex-PCR method. Materials and Methods: In this descriptive cross-sectional study was performed during a year in 2014 on the 1250 no duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of invA ،ttrC، mgtC، spi4D and agfA genes. Results: Totally, sixty S. enteritidis strains were obtained from the poultry meat (38 strains, 61.6%) and the eggs (23 strains, 38.3%), respectively. The highest and lowest prevalence of these genes were related to mgtC (51.6%) and spi4D (1.3%). Conclusion: Evaluation of virulence genes in the S. Enteritidis isolated from food samples are useful because of the incidence of the virulence elements and efficiency of M-PCR in the epidemiological study and evaluation of intraspecies genes transfer in the various samples. Keywords: Salmonella enteritidis, Food samples, Virulence genes, Multiplex PCR.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | mania jalilvand |
| Date Deposited: | 27 Dec 2016 06:16 |
| Last Modified: | 27 Dec 2016 06:16 |
| URI: | http://eprints.lums.ac.ir/id/eprint/343 |
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