Reversed-phase vortex-assisted liquid–liquid microextraction: A new sample preparation method for the determination of amygdalin in oil and kernel samples

Heydarib, Rouhollah and Hosseinia, Mohammad and Alimoradia, Mohammad (2014) Reversed-phase vortex-assisted liquid–liquid microextraction: A new sample preparation method for the determination of amygdalin in oil and kernel samples. Journal of Separation Science.

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Abstract

A novel, simple and rapid reversed-phase vortex-assisted liquid–liquid microextraction coupled with high-performance liquid chromatography has been introduced for the extraction, clean-up and pre-concentration of amygdalin in oil and kernel samples. In this technique, deionized water was used as the extracting solvent. Unlike the reversed-phase dispersive liquid–liquid microextraction, dispersive solvent was eliminated in the proposed method. Various parameters that affected the extraction efficiency, such as extracting solvent volume and its pH, vortex and centrifuging times were evaluated and optimized. The calibration curve shows good linearity (r2=0.9955) and precision (RSD<5.2 %) in the range of 0.07–20 μg mL-1. The limit of detection and limit of quantitation were 0.02 and 0.07 μg mL-1, respectively. The recoveries were in the range of 96.0–102.0 % with relative standard deviation values ranging from 4.0–5.1%. Unlike the conventional extraction methods for plant extract no evaporative and re-solubilizing operations were needed in the proposed technique. Keywords: Amygdalin; High-performance liquid chromatography; Liquid-liquid microextraction; Reversed phase ;Sample preparation;

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