A loop‑mediated isothermal amplifcation (LAMP) assay for detection of Toxoplasma gondii infection in women with spontaneous abortion

Kheirandish, Farnaz and Fallahi, Shirzad and Mahmoudvand, Hossein and Araban, Ali and Anbari, Khatereh and Karimi Rouzbahani, Arian and Akbari, Soheila (2020) A loop‑mediated isothermal amplifcation (LAMP) assay for detection of Toxoplasma gondii infection in women with spontaneous abortion. Archives of Microbiology.

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Abstract

The present study aimed to use the loop-mediated isothermal amplifcation (LAMP) technique in comparison with serological tests to determine the rate of T. gondii infection in women sufering from spontaneous abortion (SA). A total of 140 women sufering from their frst SA were included in this study. The collected aborted fetal remains and blood samples from each patient were examined in sterilized conditions using the LAMP technique and ELISA. Of the 140 women, 80 (57.1%) tested seropositive for anti-Toxoplasma antibodies by ELISA, 72 (51.4%) women tested seropositive for the IgG antibody, 8 (5.7%) tested seropositive for the IgM antibody. Among the eight women who’d had their frst SA who tested seropositive for IgM antibody by ELISA, only fve cases (62.5%) reported positively to the LAMP test. The diference in the frequency distribution of the LAMP results for measuring the Toxoplasma infection in pregnant women under study was statistically signifcant (P < 0.001) from the results of the serological test (ELISA). Although there was a signifcant diference between age and positivity in the LAMP test (P = 0.017), no signifcant diference was observed between positivity in the LAMP test and other variables. The fndings of the present investigation suggest that LAMP is a preferred method for determining Toxoplasma infection in pregnant women sufering from SA compared with other routine serological tests. Even in a feld with limited facilities and equipment, this technique can be efective and efcient in accurately and specifcally diagnosing Toxoplasma infections in women at high risk of SA.

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