Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification

Nori-Garavand, Reza and Hormozi, Maryam and Narimani, Leila and Beigi Boroujeni, Nasim and Rajabzadeh, Asghar and Zarei, Leila and Beigi Boroujeni, Masoud and Beigi Boroujeni, Mandana (2020) Effect of Selenium on Expression of Apoptosis-Related Genes in Cryomedia of Mice Ovary after Vitrification. Hindawi BioMed Research International.

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Abstract

Introduction. Freezing of ovarian tissue is used for preservation of fertility. The freezing-thawing process is accompanied by oxidative stress and induction of apoptosis. Apoptosis is a complex process that has been studied in animal models. The present study was aimed at investigating the effect of selenium on suppression of apoptosis during vitrification-thawing process of mice ovary via studying expression of apoptosis-related genes, and also, we aimed to design statistical models for the roles of single genes and gene-gene interactions in suppression of apoptosis. Methods. A total of 10 right ovary samples from 10 mice were randomly divided into two groups of selenium treatment (at dose 5 μg/ml sodium selenite, through adding to the media) and control group. Vitrification-thawing process was done according to the existed protocols. Real-time PCR was used for gene expression study. The apoptosis gene profile included P53, Bax, Fas, and Bcl-2. General linear model was applied to study single gene associations and gene-gene interactions. Results. From the studied genes, P53 showed a significant downregulation in the selenium group in comparison to the control group (ΔΔCT = 1:96; P = 0:013; relative expression ðREÞ = 0:28). Bcl-2 showed a significant upregulation in the selenium group in comparison to the control group (ΔΔCT = −2:49; P < 0:001; RE = 3:49). No significant result was found for other genes. According to the multiple models, Bcl-2 showed a protective single gene association (beta = −0:33; P = 0:032), and Fas ∗ Bcl-2 interaction was significantly positive (beta = 0:19; P = 0:036). Conclusion. Addition of selenium to cryomedia of vitrification-thawing process could reduce the apoptosis induced by freezing-thawing stress in mice ovary via downregulation of P53 and upregulation of Bcl-2 at transcription level. Multivariable statistical models should be performed in future researches to study biological systems.

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: samira sepahvandy
Date Deposited: 11 Oct 2020 04:16
Last Modified: 11 Oct 2020 04:16
URI: http://eprints.lums.ac.ir/id/eprint/2405

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