Selenium Effect on Ischemia-Reperfusion Injury of Gastrocnemius Muscle in Adult Rats

Gholami, Mohammadreza and Zendedel, Abolfazl and Khanipour khayat, Zahra and Ghanad, Kourosh and Nazari, Afshin and Pirhadi, Atieh (2014) Selenium Effect on Ischemia-Reperfusion Injury of Gastrocnemius Muscle in Adult Rats. Biol Trace Elem Res, 164.

Preview

Text 774-1093-1-SM.pdf Download (467kB) | Preview

Abstract

Abstract Selenium is a trace element that has antioxidant and neuroprotective effects. The aim of this study is to investigate the effects of selenium in reducing ischemia-reperfusion injury of the gastrocnemius muscle. In this experimental study, 80 adult male Wistar rats weighing 250–300 g were divided into ten groups (N=8 per group). Group 1 is control group (without ischemia-reperfusion). Group 2 received 0.2 mg/kg selenium. Group 3 received ischemia+3 d reperfusion+0.2 mg/kg selenium, group 4 received ischemia+3 d reperfusion+0.2mg/kg placebo, group 5 received ischemia+7 d reperfusion+ 0.2 mg/kg selenium, group 6 received ischemia+7 d reperfusion+ 0.2 mg/kg placebo, group 7 received ischemia+14 d reperfusion+0.2 mg/kg selenium, group 8 received ischemia+ 14 d reperfusion+0.2 mg/kg placebo, group 9 received ischemia+28 d reperfusion+0.2 mg/kg selenium and group 10 received ischemia+3 d reperfusion+0.2 mg/kg placebo. External iliac artery blocked for 3 h. After reperfusion, rats killed and gastrocnemius muscle removed, fixed, and tissue processing performed. Samples stained with hematoxylineosin for edema evaluation, toluidine blue for mast cell infiltration evaluation and immunohistochemistry for detection TNF-alpha and NF-kappa B proteins. Comparison of mast cell infiltration, edema of the interstitial fluid on the tissue, expression of TNF-alpha protein, and expression of NF-kappa B protein in the groups that received selenium with corresponding placebo group showed that selenium can reduce edema, mast cell infiltration, and TNF-alpha expression and inactivated NF-kappa B. The use of selenium simultaneously with creating ischemia can reduce ischemia-reperfusion injury of the gastrocnemius muscle. Keywords Ischemia-reperfusion . Muscle . TNF-alpha . NF-kappa B

Actions (login required)

View Item