Javanmard, Ehsan and Ohari, Yuma and Sadeghi, Amir and Cheraghipour, Kourosh and AsadzadehAghdaei, Hamid (2019) MultigenetypingandphylogeneticanalysisofFasciolafromendemicfociin Iran. Infection,GeneticsandEvolution.
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Abstract
Fasciolosisisapublichealthproblemoriginallytransmittedfromlivestock.Althoughmolecularanalysisofthe nuclearandmitochondrialgenesallowdiscriminationbetweenthetwoknownspeciesofFasciola,F.hepaticaand F.gigantica,thesemarkersdonotpermitthedetectionofhybridforms.Ontheotherhand,molecularanalysisof thepepckandpoldgenesfromFascioladopermitthedetectionofhybridisolatesandthisstudyhasnotyetbeen performedonspecimensfromhumananddomesticatedanimalsinIran.Therefore,theaimofthisstudywasto molecularly analyze Fasciola isolates using both conventional and the two new genetic markers mentioned above. Fifty-three adult Fasciola worms were isolated from the livers of 28 domesticated animals, including sheep,cattleandgoat.Moreover,anadultwormwasobtainedfromthebileductofaninfectedwomanduring endoscopic retrograde cholangio-pancratography (ERCP). Targeted fragments from the ITS-1, NDI and COX1 geneswereamplifiedusingspecificprimers.PCRproductsweresequencedandgeneticallyanalyzedusingMEGA v.7andDnaSPsoftware.Additionally,thepepckandpoldregionswereamplifiedandanalyzedusingmultiplex PCRandRLFP-PCR,respectively.Multiplealignmentofsequencedfragmentsshowedhighestsimilarityamong the ITS-1 sequences isolated from all four hosts in comparison to the other genes. Furthermore, sequence diversityacrosstheCOX1washigherthantheNDIandITS-1.Diversityamongsequencesisolatedfromcattlewas higherthanthosefromsheepandgoatinallthreegenes.Tajima'sDandFu'sFswerenegativeandstatistically significantforallthegenesexceptITS-1.PhylogenetictreesshowedthatthehumanF.hepaticaisolatewascloser tosheepisolates.TheresultsofthepepckandpoldanalysesshowedthatallisolateswereF.hepaticaandthere werenohybridformsamongsamples.Themolecularanalysescorroboratedthisfinding.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 09 Feb 2020 05:09 |
| Last Modified: | 09 Feb 2020 05:09 |
| URI: | http://eprints.lums.ac.ir/id/eprint/1932 |
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