ComparisonoftheRE-529sequenceandB1geneforToxoplasmagondii detectioninbloodsamplesoftheat-riskseropositivecasesusinguracilDNA glycosylasesupplementedloop-mediatedisothermalamplification(UDGLAMP)assay

Keshavarz Valian, Hossein and Mirhendi, Hossein and Mohebali, Mehdi and Shojaee, Saeedeh and Fallahi, Shirzad and Kheirandish, Farnaz (2019) ComparisonoftheRE-529sequenceandB1geneforToxoplasmagondii detectioninbloodsamplesoftheat-riskseropositivecasesusinguracilDNA glycosylasesupplementedloop-mediatedisothermalamplification(UDGLAMP)assay. MicrobialPathogenesis.

[img]
Preview
Text
1-s2.0-S0882401019315128-main.pdf

Download (834kB) | Preview

Abstract

Diagnosisoftoxoplasmosisisanimportantissue,especiallyinat-riskpatients.Themolecularmethodsshoweda promising future for such diagnosis; however, the method itself and the target sequence to be detected is an importantpartofaccuratedetectionoftheinfection.TheaimofthepresentstudywastoevaluatetheRE-529 sequenceandB1geneforToxoplasmagondiidetectioninbloodsamplesoftheat-riskseropositive casesusing uracilDNAglycosylasesupplementedloop-mediatedisothermalamplification(UDG-LAMP)assay.Inthisstudy, 110 T. gondii seropositive at-risk individuals (pregnant women and immunocompromised patients) and 110 seronegativecontrolswereenrolled.Thetwomoststudiedsequences(RE-529andB1)wereusedandcompared foraccurateandreliabledetectionofT.gondiiinbloodsamplesusingUDG-LAMPassayandcomparedwithrealtimePCRmethod.Thedetectionlimit,accuracy,andreliabilityofUDG-LAMPfortheparasite'sDNAwerealso studied.Among110studiedcases,39(35.45%)and36(32.7%)werepositiveforT.gondiiDNAwiththeRELAMPandB1-LAMP,respectively.TheseronegativecasesremainednegativeforT.gondiiDNAwiththestudied genes,however,therewerefewfalsenegativescomparedwithreal-timePCRmethod.Thedetectionlimitofthe UDG-LAMPforbothDNAtargetswas0.16tachyzoite'sDNAperreactiontube.Basedontheresultsofthisstudy, the RE-529 sequence has a better detection rate compared to the B1 gene for toxoplasmosis among at-risk people. UDG-LAMP is a highly sensitive, accurate, and reliable method with no false-positive results for the diagnosisofT.gondiiinfectioninbloodspecimens,howeverfewcasesmaybemissed

Item Type: Article
Subjects: R Medicine > RB Pathology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: samira sepahvandy
Date Deposited: 09 Feb 2020 04:49
Last Modified: 09 Feb 2020 04:49
URI: http://eprints.lums.ac.ir/id/eprint/1930

Actions (login required)

View Item View Item