Daneshmandi, Saeed and Shahrokhi, Somayeh (2019) Engineering Tumor Cells with Tumor Necrosis Factor alpha (TNF-alpha) or CD40 Ligand (CD40L) Genes Induce Anti-tumor Immune Responses. INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, 25 (2). pp. 427-436.
Full text not available from this repository.Abstract
One of the main problems in tumor therapy is immunosuppressive microenvironment of the tumor. To overcome this, we assessed targeted tumor cells with tumor necrosis factor- (TNF-) or CD40 Ligand (CD40L) to improve antigen presenting cells function and subsequently anti-tumor responses. 4T1 tumor cells were transfected with TNF- and CD40L genes using lipofectamine and chitosan nanocomplexes. Engineered tumor cells were assessed in vitro and in vivo. Results showed that chitosan nanoparticles delivery of TNF- or CD40L to 4T1 co-cultured with DCs induced expression of DCs co-stimulatory markers and enhanced the secretion of pro-inflammatory mediators IL-6, TNF- and IL-12. The DCs were also able to enhance expansion of T cells with enhanced IFN- and decreased IL-4 production. TNF- or CD40L engineered 4T1 cells resulted into delay in tumor growth. The study shows that nanoparticle manipulation of tumor cells by TNF- or CD40L induce anti-tumor immune responses. Then, strategies that apply chitosan nanoparticles could provide a potent tool for tumor targeting and treatments.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RC Internal medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 20 May 2019 06:25 |
| Last Modified: | 20 May 2019 06:25 |
| URI: | http://eprints.lums.ac.ir/id/eprint/1713 |
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