Novel Antioxidant Properties of Ghrelin and Oleuropein Versus Lipopolysaccharide-Mediated Renal Failure in Rats

Alirezaei, Masoud and Dezfoulia, Omid and Kheradmand, Arash (2015) Novel Antioxidant Properties of Ghrelin and Oleuropein Versus Lipopolysaccharide-Mediated Renal Failure in Rats. Int J Pept Res The. pp. 411-421.

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Abstract

Ghrelin and oleuropein have recently been shown to bear beneficial effects against oxidative stress in our reports. The aim of this study was to evaluate the antioxidant abilities of ghrelin and oleuropein in a lipopolysaccharide (LPS)-induced nephrotoxicity model. The Sprague-Dawley male rats were divided into LPS, the ghrelin1 plus LPS (Ghr1?LPS), the ghrelin4 plus LPS (Ghr4?LPS), the oleuropein10 plus LPS (Ole10?LPS) and the oleuropein15 plus LPS (Ole15?LPS) groups. 1 or 4 nmol/ rat of ghrelin as subcutaneous and oleuropein 10 or 15 mg/kg as oral were administrated to ghrelin and oleuropein pretreated rats and vehicle injected to LPS group for ten consecutive days. All of the groups received LPS (once; 5 mg/ kg) in the 11th day of the treatment as intraperitoneally. LPSinduced nephrotoxicity was manifested by a significant elevation in renal function tests (BUN and creatinine; P\ 0.001) and histopathology findings in LPS group in comparison with the ghrelin and oleuropein pretreated rats. Renal lipid peroxidation was significantly higher in the LPStreated animals than the ghrelin and oleuropein pretreated groups (P\ 0.05). In contrast, glutathione peroxidase (GPx), catalase and superoxide dismutase activities were significantly higher in Ghr4?LPS-treated rats than LPS group (P\ 0.05). GPx activity was also significantly higher in Ole15?LPS-treated rats than LPS group (P\ 0.05). Regarding nitrosative stress, renal nitrite content was significantly lower in Ghr4?LPS and Ole15?LPS groups than LPS group (P\ 0.05). These results suggest that ghrelin and oleuropein have beneficial antioxidant properties versus LPS-induced renal failure in rats. Keywords Ghrelin � Oleuropein � Lipopolysaccharide � Nephrotoxicity � Oxidative stress

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