Evaluation of AD-MSC (Adipose derived –Mesenchymal stem cells) as a vehicle for IFN-β delivery in Experimental Autoimmune Encephalomyelitis

Mohammadzadeh, Adel and Pourfathollah, Ali Akbar and Shahrokhi, Somayeh and Fallah, ali and Tahoori, Mohammad Taher and Amari,, Afshin and Forouzandeh, Mahdi and Soleimani, Masoud (2016) Evaluation of AD-MSC (Adipose derived –Mesenchymal stem cells) as a vehicle for IFN-β delivery in Experimental Autoimmune Encephalomyelitis. Clinical Immunology.

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Abstract

Interferon-β (IFN-β) is commonly used as a disease modifying drug for the treatment of Relapseremitting multiple sclerosis (RR-MS). However, the underlying mechanism by which IFN-β mediate this immunosuppressive effect is still unknown. In this study, we analyzed the effects of genetically modified Adipose Derived Mesenchymal Stem Cells (AD-MSCs) expressing murine interferon beta (MSCs-VP/IFN-β) on the animal model of MS, experimental autoimmune encephalomyelitis (EAE). Lymph node mononuclear cells and serum were examined by using RT-PCR and ELISA methods to measure the production of IL-10 and IL-17 gene and protein expression, respectively. Our results indicated that in the MSCs-VP/IFN-β treated group induction of Tregs and IL-10 and reduction of IL-17 were significant. Taken together, we showed that using AD-MSCs expressing IFN-β as an anti-inflammatory agent, offer evidence supporting that the stem cell therapies in EAE conceivably will improve the valuable effects of IFN-β in this autoimmune disease. Key words; MSCs (Mesenchymal Stem Cells), IFN-β, Treg, EAE

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: sobhan rezaiian
Date Deposited: 08 May 2017 05:08
Last Modified: 18 Nov 2017 15:37
URI: http://eprints.lums.ac.ir/id/eprint/568

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