Effect of Six Weeks of Aerobic Training with Quercetin Supplementation on the Expression of Mitochondrial AMPK and NRF-1 Genes in the Hippocampus of Diabetic Male Rats

Mohammadi, Raha and Bolboli*, Lotfali and Pourvaghar, Mohamad javad and Afroundeh, Roghayeh (2023) Effect of Six Weeks of Aerobic Training with Quercetin Supplementation on the Expression of Mitochondrial AMPK and NRF-1 Genes in the Hippocampus of Diabetic Male Rats. Yafteh, 24 (4).

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Abstract

Background: The most common neurological complication of hyperglycemia is neuropathy which leads to changes in the central nervous system. Therefore, this study aimed to investigate the effect of six weeks of aerobic exercise with quercetin supplementation on the expression of mitochondrial Ampk and Nrf-1 genes in the hippocampus of diabetic male rats. Materials and Methods: This experimental study was performed by the laboratory method. Thus, 35 male Wistar rats were divided into five groups (n=7): Healthy Control, Diabetes Control, Diabetes Exercise, Diabetes Exercise Quercetin Supplement, and Quercetin Supplement. During the course, the supplement and diabetic groups were injected with 15 mg/kg of quercetin per day after induction of diabetes. For the training groups, six weeks of moderate-intensity aerobic exercise was performed on a revolving bar. To evaluate the expression of mitochondrial Ampk and Nrf-1 genes, hippocampal tissue was isolated 24 h after the last training session. The SPSS statistical software (version 23), one-way analysis of variance (ANOVA), and Tukey's post hoc test onther significance level of 0.05 were used to analyze the collected data. Result: The findings of this study showed that after six weeks of aerobic exercise, the expression of mitochondrial Ampk and Nrf-1 genes in the exercise supplement diabetes group significantly increased (P<0.05) compared to diabetes control, exercise diabetes, and quercetin supplementation. A significant increase was observed only for Ampk in the exercise diabetic group compared to the diabetic control and quercetin diabetic groups (P<0.05). Conclusion: It seems that six weeks of aerobic exercise with quercetin supplementation can positively affect the expression of mitochondrial Ampk and Nrf-1 genes in hippocampal tissue and increase it in diabetic conditions. Keywords: Aerobic exercise, Ampk, Mitochondria, Nrf-1, Quercetin.

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