Evaluation of virulence genes in the Salmonella enteritidis isolated from food ‎samples by multiplex-PCR

Mirzadeh, Sedigheh and Mahmoudi*, Razagh and Amini, Kiumars (2016) Evaluation of virulence genes in the Salmonella enteritidis isolated from food ‎samples by multiplex-PCR. Yafte, 18 (3).

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Abstract

Background : Salmonella enterica serotype Enteritidis is one of the most important cause of non-typhoidal ‎salmonellosis, a clinically less severe infection than typhoid fever. The present study aimed to ‎detection of virulence genes in the S. enteritidis isolated from the food samples by multiplex-PCR ‎method.‎ Materials and Methods: In this descriptive cross-sectional study was performed during a year in 2014 on the 1250 no ‎duplicative and non-repetitive food samples. M-PCR assay was done in order to detection of ‎invA ‎،ttrC،‎ mgtC،‎ spi4D and agfA genes.‎ Results: Totally, sixty S. enteritidis strains were obtained from the poultry meat (38 strains, 61.6%) and ‎the eggs (23 strains, 38.3%), respectively. The highest and lowest prevalence of these genes were ‎related to mgtC (51.6%) and spi4D (1.3%).‎ Conclusion: Evaluation of virulence genes in the S. Enteritidis isolated from food samples are useful because ‎of the incidence of the virulence elements and efficiency of M-PCR in the epidemiological study ‎and evaluation of intraspecies genes transfer in the various samples.‎ Keywords: Salmonella enteritidis, Food samples, Virulence genes, Multiplex PCR.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Samira Sepahvandy
Date Deposited: 27 Dec 2016 06:16
Last Modified: 27 Dec 2016 06:16
URI: http://eprints.lums.ac.ir/id/eprint/343

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