Keshavarz Valian, Hossein and Mirhendi, Hossein and Mohebali, Mehdi and Shojaee, Saeedeh and Fallahi, Shirzad and Kheirandish, Farnaz (2019) ComparisonoftheRE-529sequenceandB1geneforToxoplasmagondii detectioninbloodsamplesoftheat-riskseropositivecasesusinguracilDNA glycosylasesupplementedloop-mediatedisothermalamplification(UDGLAMP)assay. MicrobialPathogenesis.
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Abstract
Diagnosisoftoxoplasmosisisanimportantissue,especiallyinat-riskpatients.Themolecularmethodsshoweda promising future for such diagnosis; however, the method itself and the target sequence to be detected is an importantpartofaccuratedetectionoftheinfection.TheaimofthepresentstudywastoevaluatetheRE-529 sequenceandB1geneforToxoplasmagondiidetectioninbloodsamplesoftheat-riskseropositive casesusing uracilDNAglycosylasesupplementedloop-mediatedisothermalamplification(UDG-LAMP)assay.Inthisstudy, 110 T. gondii seropositive at-risk individuals (pregnant women and immunocompromised patients) and 110 seronegativecontrolswereenrolled.Thetwomoststudiedsequences(RE-529andB1)wereusedandcompared foraccurateandreliabledetectionofT.gondiiinbloodsamplesusingUDG-LAMPassayandcomparedwithrealtimePCRmethod.Thedetectionlimit,accuracy,andreliabilityofUDG-LAMPfortheparasite'sDNAwerealso studied.Among110studiedcases,39(35.45%)and36(32.7%)werepositiveforT.gondiiDNAwiththeRELAMPandB1-LAMP,respectively.TheseronegativecasesremainednegativeforT.gondiiDNAwiththestudied genes,however,therewerefewfalsenegativescomparedwithreal-timePCRmethod.Thedetectionlimitofthe UDG-LAMPforbothDNAtargetswas0.16tachyzoite'sDNAperreactiontube.Basedontheresultsofthisstudy, the RE-529 sequence has a better detection rate compared to the B1 gene for toxoplasmosis among at-risk people. UDG-LAMP is a highly sensitive, accurate, and reliable method with no false-positive results for the diagnosisofT.gondiiinfectioninbloodspecimens,howeverfewcasesmaybemissed
| Item Type: | Article |
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| Subjects: | R Medicine > RB Pathology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | lorestan university |
| Date Deposited: | 09 Feb 2020 04:49 |
| Last Modified: | 09 Feb 2020 04:49 |
| URI: | http://eprints.lums.ac.ir/id/eprint/1930 |
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