The effect of endurance activity on the HDACA4 and MEF2 gene expression in skeletal muscles of male Wistar rats

Bahrami*, Farid and Fathi, Mohamad and Ahmadvand, Hasan and Pajohi, Naser (2018) The effect of endurance activity on the HDACA4 and MEF2 gene expression in skeletal muscles of male Wistar rats. Yafte, 20 (1).

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Abstract

Background : Skeletal muscles are composed of various contracted fibrils, which are mainly divided into fast-twitch and slow-twitch. This study aimed to investigate 8 weeks endurance activity on the MEF2 and HDACA4 gene expression in fast-twitch and slow-twitch skeletal muscles in male Wistar rats. Materials and Methods: in order to carry out this study, 20 heads of male Wistar rats, age 4 weeks (110± 10), were bought from the Razi Institute of Lorestan Medical University. The same laboratory conditions were provided for the rats for the completion of 14 days of an endurance familiarization course to teach running on treadmill. At the end of this course, the rats were randomly divided into 2 groups. Experimental group (n=10 head) and control group (n= 10 head). An eight week endurance program, 5 sessions per week, was performed for the experimental group. Results: this study showed that there was no significant change in the relative gene expression of HDACA4 and MEF2 in EDL muscle in either group (P>0.05). However, the relative gene expression of MEF2 in the experimental group was not statically significant in comparison to the control group (P>0.05). In sol muscles, there was no statically significant changes in either group’s gene expression. The relative gene expression of MEF2 in the experimental group showed a statistically significant reduction in comparison to the control group (P>0.05). Conclusion: in summary, the results of this research have shown that doing 8 weeks endurance exercises did not cause any changes in HDAC4 and MEF2 gene expression in EDL muscle. Although in the SOL muscle, MEF2 gene expression decreased, no changes in the level of HDAC4 gene expression were observed. Keywords: Endurance activity, MEF2 gene, HDACA4 gene, Slow – twitch and fast- twitch muscles

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