Cardiac failure detection in 30 minutes: new approach based on gold nanoparticles

Namdari, Mehrdad and Negahdari, Babak and Cheraghi, Mostafa and Aiyelabegan, Hammed T and Eatmadi, Ali (2017) Cardiac failure detection in 30 minutes: new approach based on gold nanoparticles. JOURNAL OF MICROENCAPSULATION, 34. pp. 132-139. ISSN 0265-2048

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Abstract Cardiac failure occurs when heart is unable to pump sufficiently to maintain blood flow to meet the body's needs. The aim of this work is to detect highly expressed genes: follistatin-related protein 1 (FSTL1) in heart failure within 30 minutes, using gold nanoparticles. Gold nanoparticles were prepared by citrate reduction of HAuCl4 3H(2)O; probe sequence was designed based on the FSTL1 gene region. Preparation of gold nanoprobes (AuNPs) proceeded by treating all the containers with DEPC-treated water, followed by reduction and conjugation. Transmission electron microscopy shows that AuNPs were 10-15 nm in size. The concentration of the nanoprobes was 2.1 nM, and they bind to target. Real-time PCR shows an over-expression of FSTL1 and FSTL3 in heart failure (p<.05). Our data showed that elevated expression of the FSTL1 and FSTL3 is a marker of heart failure as detected within 30 minutes by the synthesised AuNPs; the method is accurate and fast. Keywords Author Keywords:Cardiac failure; gold nanoparticles; FSTL1; nanoprobes

Item Type: Article
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: sobhan rezaiian
Date Deposited: 23 Oct 2017 12:14
Last Modified: 23 Oct 2017 17:52

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