Assessment of the cytotoxicity and in vivo anti-Plasmodial activity of ethanol and dichloromethane extracts of four different Iraninan propolis

Afrouzan, Hoshang and Abouie Mehrizi, Akram and Shokrgozar, Mohamad Ali and Tahghighi, Azar and Es-haghi, Ali and Dinparast Dgadid, Navid and Zakeri*, Sedigheh (2017) Assessment of the cytotoxicity and in vivo anti-Plasmodial activity of ethanol and dichloromethane extracts of four different Iraninan propolis. Yafte, 19 (2).

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Abstract

Background: The emergence of antimalarial drug resistance is one of the great problems of malaria control and elimination of worldwide. In order to overcome anti-malarial drug resistance, cytotoxicity and in vivo anti-Plasmodiumal activity of four different Iranian propolis were investigated. Materials and Methods: Four Iranian propolis samples were collected from four different regions of Iran and extracted by %70 ethanol and dichloromethane. The cytotoxicity of ethanolic and dichloromethane extract of propolis, using L969 fibroblast cell lines, were evaluated by MTT assay. The in vitro anti-Plasmodial activities of the propolis samples on BALB/c mice were assayed. Results:The cytotoxicity results showed that ethanol and dichloromethane extracts of four Iranian propolis samples at doses of 25, 50, 100 and 200 µg/ml were non-toxic (P<0.05). The highest percentage of growth inhibition against Plasmodium berghei with %71 and %65 was for ethanol and dichloromethane extract of Morad Byge propolis, respectively. Conclusion: Considering the drug resistance of P. falciparum to conventional medicine and its dangers, and the emergence of development cheap and safe anti-malaria drugs to control and eliminate programs. Further investigation on Iranian propolis as safe and anti-malarial drug is recommended. Keywords: Iran, Propolis, Cytotoxicity, Anti-plasmodium.

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