The effect of aloe vera on the expression of wound healing factors (TGFb1 and bFGF) in mouse embryonic fibroblast cell: In vitro study

Hormozi, Maryam and Assaei, Raheleh and Beigi Boroujeni, Mandana (2017) The effect of aloe vera on the expression of wound healing factors (TGFb1 and bFGF) in mouse embryonic fibroblast cell: In vitro study. Biomedicine & Pharmacotherapy, 88. pp. 610-616.

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Abstract

Background: Aloe vera (A.v) have been used traditionally for topical treatment of wounds and burns in different countries for centuries, but the mechanism of this effect is not well understood. Various growth factors are implicated in the process of wound healing. Among the different growth factors involved in the process, TGFb1 and bFGF are the most importantly expressed in fibroblast cells. The aim of this study was to evaluate the effect of A.v on the expression of angiogenesis growth factors in mouse embryonic fibroblast cells. Methods: We exposed mouse embryonic fibroblast cells to different concentrations of A.v (50, 100 and 150 mg/ml) at two different time of 12 and 24 h. Fibroblast cell without A.v treatment serves as the control. The expression of TGFb1and bFGF was measured by real time-polymerase chain reaction (real- time-PCR) and enzyme-linked immunosorbent assay (ELISA) at the level of gene and protein. Results: We observed that A.v gel at first up-regulated the expression of TGFb1 and bFGF, but, these genes were later repressed after a particular time. Conclusion: Our results demonstrated that A.v was dose-dependent and time-dependent on the expression of bFGF and TGFb1 in fibroblast cell in vitro. This mechanism can be employed in the prospective treatment of physical lesion.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: sobhan rezaiian
Date Deposited: 23 Oct 2017 12:10
Last Modified: 23 Oct 2017 12:10
URI: http://eprints.lums.ac.ir/id/eprint/800

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