Considerable Genetic Diversity of Trichomonas vaginalis Clinical Isolates in a Targeted Population in South of Iran

TAVAKOLI OLIAEE, Razieh and BABAEI, Zahra and HATAM, Gholam Reza and TAVAKOLI KARESHK, Amir and MAHMOUDVAND, Hosein and VAFAFAR, Arghavan and ZIAALI, Naser (2017) Considerable Genetic Diversity of Trichomonas vaginalis Clinical Isolates in a Targeted Population in South of Iran. Iran J Parasitol, 12 (2). pp. 251-259.

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Abstract

Abstract Background: The present study aimed to characterize genetically and to compare the most frequently occurring strains of Trichomonas vaginalis isolated from southern Iran. Methods: Totally, 150 vaginal swab and urine specimens were collected from symptomatic and asymptomatic women from May 2012 to Jun 2013.This study implemented a sensitive and reliable PCR-restriction fragment length polymorphism (RFLP) typing method on the actin gene. Moreover, one representative sample of each identified genotype was subjected to sequencing. Results: Twenty-four T. vaginalis isolates were positive and6 distinct electrophoretic patterns (H, E, G, I, M, N) were identified. Genotypes H and I were found to be more prevalent (50 and 37.5%) in Kerman and Shiraz, respectively. The phylogenetic analysis showed that two isolates were located as a separated clade with the other T. vaginalis isolates. Conclusion: The obtained findings showed a considerable genetic polymorphism of clinical isolates from the population studied. More studies may be warranted in future as to unveiling any possible links between a given genotype/cluster and pathogenic behavior of T. vaginalis.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: sobhan rezaiian
Date Deposited: 23 Oct 2017 12:09
Last Modified: 23 Oct 2017 12:09
URI: http://eprints.lums.ac.ir/id/eprint/798

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