Azadpour, m and Nowroozi, J and Goudarz, gr and Mahmoudvand, h (2015) Presence of qacE Delta 1 and cepA genes and susceptibility to a hospital biocide in clinical isolates of Klebsiella pneumoniae in Iran. TROPICAL BIOMEDICINE, 32 (1). pp. 109-115. ISSN 0127-5720
Full text not available from this repository.Abstract
The aim of this study was to evaluate the susceptibility of Klebsiella pneumoniae clinical isolates to antibiotics and to a quaternary ammonium compound (QAC) disinfectant as the concentrations used clinically and to determine the presence of the qacE Delta 1 and cepA genes for the first time in Iran. In total, 85 K. pneumoniae isolates were randomly collected from hospitalized patients at the general hospitals in Lorestan, Iran. Antibiotic and antiseptic susceptibility testing was performed according to Clinical and Laboratory Standards Institute recommendations. K. pneumonia isolates were screened by PCR amplification of qacE Delta 1 and cepA genes using specific primers and sequence analysis of the amplified regions were also performed. From 85 isolates of K. pneumoniae, 34 (40%) isolates were multidrug resistance (MDR). The evaluation of the susceptibility to the QAC disinfectant revealed that 51 (60%) isolates had reduced susceptibility to QAC disinfectant. The qacE Delta 1 gene was detected in 26 isolates (30.6%). While cepA gene was found in 19 isolates (22.3%) of K. pneumonia. Seventy-three percent (19/26) qacE Delta 1-positive isolates were detected in the biocide-resistant isolates. Whereas, 63.1% (12/19) cepA-positive isolates were found in the biocide-resistant isolates. Out of qacE Delta 1 and cepA-positive isolates, 65.4% (17/26) and 42.1% (8/19) were among MDR isolates, respectively. No significant association of biocide resistance with the presence of qacE Delta 1 and cepA genes was observed (P>0.05). The results of present study shows that there was a close link between qacE Delta 1 gene and antibiotic resistance, but no significant association of biocide resistance with the presence of qacE Delta 1 and cepA genes was observed in K. pneumoniae in Iran.
| Item Type: | Article |
|---|---|
| Subjects: | G Geography. Anthropology. Recreation > GB Physical geography |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 23 Oct 2017 12:07 |
| Last Modified: | 23 Oct 2017 12:07 |
| URI: | http://eprints.lums.ac.ir/id/eprint/731 |
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