Selenium Effect on Ischemia-Reperfusion Injury of Gastrocnemius Muscle in Adult Rats

Gholami,, Mohammadreza and Zendedel, Abolfazl and (Khayat,, Zahra Khanipour)[ and Ghanad,, Kourosh and Nazari,, Afshin and Pirhadi,, Atieh) (2015) Selenium Effect on Ischemia-Reperfusion Injury of Gastrocnemius Muscle in Adult Rats. BIOLOGICAL TRACE ELEMENT RESEARCH, 164 (2). pp. 205-211. ISSN 0163-4984

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Abstract

Selenium is a trace element that has antioxidant and neuroprotective effects. The aim of this study is to investigate the effects of selenium in reducing ischemia-reperfusion injury of the gastrocnemius muscle. In this experimental study, 80 adult male Wistar rats weighing 250-300 g were divided into ten groups (N = 8 per group). Group 1 is control group (without ischemia-reperfusion). Group 2 received 0.2 mg/kg selenium. Group 3 received ischemia + 3 d reperfusion + 0.2 mg/kg selenium, group 4 received ischemia + 3 d reperfusion + 0.2 mg/kg placebo, group 5 received ischemia + 7 d reperfusion + 0.2 mg/kg selenium, group 6 received ischemia + 7 d reperfusion + 0.2 mg/kg placebo, group 7 received ischemia + 14 d reperfusion + 0.2 mg/kg selenium, group 8 received ischemia + 14 d reperfusion + 0.2 mg/kg placebo, group 9 received ischemia + 28 d reperfusion + 0.2 mg/kg selenium and group 10 received ischemia + 3 d reperfusion + 0.2 mg/kg placebo. External iliac artery blocked for 3 h. After reperfusion, rats killed and gastrocnemius muscle removed, fixed, and tissue processing performed. Samples stained with hematoxylin-eosin for edema evaluation, toluidine blue for mast cell infiltration evaluation and immunohistochemistry for detection TNF-alpha and NF-kappa B proteins. Comparison of mast cell infiltration, edema of the interstitial fluid on the tissue, expression of TNF-alpha protein, and expression of NF-kappa B protein in the groups that received selenium with corresponding placebo group showed that selenium can reduce edema, mast cell infiltration, and TNF-alpha expression and inactivated NF-kappa B. The use of selenium simultaneously with creating ischemia can reduce ischemia-reperfusion injury of the gastrocnemius muscle.

Item Type: Article
Subjects: M Music and Books on Music > MT Musical instruction and study
Depositing User: sobhan rezaiian
Date Deposited: 23 Oct 2017 12:07
Last Modified: 23 Oct 2017 12:07
URI: http://eprints.lums.ac.ir/id/eprint/720

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