Satureja khuzestanica prevents the development of morphine analgesic tolerance through suppression of spinal glial cell activation in rats

Esmaeili-Mahani, Saeed) and Ebrahimi,, Bahareh and Abbasnejad, Mehdi and Rasoulian, Bahram and Sheibani,, Vahid)[ (2015) Satureja khuzestanica prevents the development of morphine analgesic tolerance through suppression of spinal glial cell activation in rats. JOURNAL OF NATURAL MEDICINES, 69 (2). pp. 165-170. ISSN 1340-3443

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Abstract

Glial cell activation and oxidative stress are important factors in the induction of opioid side effects such as tolerance and dependence. It has been demonstrated that Satureja khuzistanica extract (SKE) has antioxidative, antinociceptive and anti-inflammatory properties; however, its influences on opioid analgesic tolerance have not yet been clarified. Adult male Wistar rats were rendered analgesic-tolerant by injection of 10 mg/kg morphine twice daily for 8 days. To determine the effect of SKE on the development of morphine tolerance, different doses of SKE (25, 50 and 100 mg/kg i.p.) were injected simultaneously with morphine. The tail-flick test was used to assess the nociceptive threshold. The lumbar spinal cord was assayed to determine glial fibrillary acidic protein (GFAP) and tumor necrosis factor alpha (TNF alpha) levels by the Western blotting method. Our results showed that chronic morphine produced tolerance to the antinociceptive effect of morphine. However, SKE could prevent, in a dose-dependent manner, morphine tolerance development. In tolerant animals, a significant increase in GFAP and TNF alpha levels was observed in the lumbar spinal cord, and was reversed to control levels by 100 mg/kg SKE. Our data revealed that Satureja khuzistanica had beneficial effects in preventing opioid tolerance and the underlying mechanisms of those effects may be due, at least in part, to reduce spinal glial cell activation. Keywords

Item Type: Article
Subjects: C Auxiliary Sciences of History > CB History of civilization
Depositing User: sobhan rezaiian
Date Deposited: 23 Oct 2017 12:07
Last Modified: 23 Oct 2017 12:07
URI: http://eprints.lums.ac.ir/id/eprint/718

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