Vasopressin attenuates ischemia-reperfusion injury via reduction of oxidative stress and inhibition of mitochondrial permeability transition pore opening in rat hearts

Nazari, Afshin) and Sadr,, Seyed Shahabeddin and (Faghihi,, Mahdieh and Azizi,, Yaser and Hosseini, Mir-Jamal) and Mobarra, Naser and Tavakoli,, Asadollah) and Imani,, AliReza (2015) Vasopressin attenuates ischemia-reperfusion injury via reduction of oxidative stress and inhibition of mitochondrial permeability transition pore opening in rat hearts. EUROPEAN JOURNAL OF PHARMACOLOGY, 760 (1). pp. 96-102. ISSN 0014-2999

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Abstract

Aim of this study was to investigate the involvement of the mitochondrial permeability transition pore (MPTP) and oxidative stress in the cardioprotective effect of vasopressin (AVP) on ischemia/reperfusion (I/R) injury. Anesthetized male wistar rats were subjected to regional 30 min ischemia and 120 min reperfusion and randomly divided into nine groups: (1) Control; saline was administered intravenously before ischemia, (2) vasopressin was administrated 10 min prior to ischemia, (3,4) Atractyloside as MPTP opener, was injected 5 min prior to reperfusion without and with vasopressin, (5, 6) Cyclosporine A as a MPTP closer, was injected 5 min prior to reperfusion without and with vasopressin, (7) mitochondria were isolated from control group and CaCl2 was added as MPTP opener and swelling inducer, (8) isolated mitochondria from Control hearts was incubated with Cyclosporine A before adding the CaCl2 (9) CaCl2 was added to isolated mitochondria from vasopressin group. Infusion of vasopressin decreased infarct size (18.6 +/- 1.7% vs. control group 37.6 +/- 2.4%), biochemical parameters [LDH (Lactate Dehydrogenase), CK-MB (Creatine Kinase-MB) and MDA (Malondialdehycle) plasma levels, PAB (Prooxidant-antioxidant balance)] compared to control group. Atactylosicle suppressed the carclioprotective effect of vasopress in (32.5 +/- 1.9% vs. 18.6 +/- 1.7%) but administration of the Cyclosporine A without and with vasopressin significantly reduced infarct size to 17.7 +/- 4% (P<0.001) and 22.7 +/- 3% (P < 0.01) respectively, vs. 37.6 +/- 24% in control group. Also vasopressin, similar to Cyclosporine A, led to decrease in CaCl2-induced swelling It seems that vasopressin through antioxidant effect and MPTP inhibition has created a cardioprotection against ischemiaireperfusion injuries. (C) 2015 Elsevier B.V. All rights reserved.

Item Type: Article
Subjects: L Education > LE Individual institutions (America except United States)
Depositing User: sobhan rezaiian
Date Deposited: 23 Oct 2017 12:04
Last Modified: 23 Oct 2017 12:04
URI: http://eprints.lums.ac.ir/id/eprint/687

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