Betaine improves gastroprotective effects of ranitidine and omeprazole against Indomethacin-induced gastric ulcer in rats

Shahsavari, Gholamreza and Alirezaei, Masoud and Jaldani, Vahid and Dezfoulian, Omid (2017) Betaine improves gastroprotective effects of ranitidine and omeprazole against Indomethacin-induced gastric ulcer in rats. Herbal Medicines Journal, 2 (1). pp. 9-17. ISSN 2538-2144

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Abstract

Abstract Background and Aim: Antioxidant capacity of betaine has been indicated in our recent studies. Thus examined oral betaine as an antioxidant agent in combination with antisecretory drugs to prevent indometha induced gastric damages in rats. Materials and Methods: Fifty-six adult male Sprague–Dawley rats were divided into two controls (nega and normal) and five experimental groups as follows: betaine-indomethacin (Bet.-Ind.), ascorbic a indomethacin (Asc.-Ind.), omeprazole-indomethacin (Ome.-Ind.), betaine-omeprazole plus indomethacin (B Ome.-Ind.) and betaine-ranitidine plus indomethacin (Bet.-Ran.-Ind.). Results: The betaine pretreated groups received betaine at a dosage of 1.5% (w/w) in their diet, wherea mg/kg of ascorbic acid was administered orally to the Asc.-Ind., group for 15 consecutive days. After a 24 fast, all the groups received 48 mg/kg of indomethacin once except for normal control group. The omepra and ranitidine groups also received one dose of omeprazole (10 mg/kg) and ranitidine (50 mg/kg), 120 min before receiving indomethacin. Histopathological findings indicated the gastroprotective effects of betaine ranitidine in pretreated rats. Pretreatment by betaine and ranitidine increased significantly the ulcer in inhibition (%), in comparison with ascorbic acid and omeprazole (alone) treatment. Glutathione peroxi (GPx) activity was significantly higher in the Bet.-Ran.-Ind., group as compared to the Asc.-Ind., and O Ind., treated rats. GPx activity also increased significantly in Bet. -Ind., treated rats as compared to the Asc. group. Catalase (CAT) activity was remarkably higher in the Bet.-Ran.-Ind., treated rats than the Asc.-Ind. Ome.-Ind., groups. TBARS concentration as a lipid peroxidation marker increased significantly in Ome. - group as compared to the Bet.-Ind., and Bet.-Ran.-Ind., treated rats. Conclusion: Thus, it seems that betaine as an antioxidant agent, is able to improve the effects of ranitidine omeprazole against indomethacin-mediated gastric damages in rats. It may also be promising in the preven of NSAIDs side effects. Keywords: Betaine, Indomethacin, Omeprazole, Ranitidine, Ulcer, Rat

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