Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells

Rezaei, Maryam and Kaeidi, Ayat and Rashidipour, Marzieh (2016) Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells. Herbal Medicines Journal, 1 (1). pp. 18-23. ISSN 2538-2144

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Abstract

Abstract Background and Aim:The protective effect of oleuropein,a chief polyphenol compound in olive tree, on cisplatin-induced nephrotoxicity in human cultured renal tubular cells, GP-293, was investigated. Materials and Methods:Cell viability was determined by the MTT assay. Cleaved caspase-3 and Bax: Bcl2 ratioas biochemical parameters of cellular apoptosis, was assessed using western blot analysis. Results:Our data showed that cisplatin in the dose of 55µg/ml significantly can reduced cell viability, increased caspase-3 activation and Bax: Bcl2 ratio in GP-293 cells after 24hr. Incubation of the cisplatin treated cells, with 20μg/ml oleuropein decreased the cisplatin-induced cell toxicity, as well as prevented caspase-3 activation and reduced Bax: Bcl2 elevation ratio. Conclusions: Oleuropein protects against cisplatin-induced cellular toxicity. This could be associated with the prevention of cellular apoptosis. Keywords:Cisplatin, Nephrotoxicity, Apoptosis, GP-293 cells, Oleuropein

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: sobhan rezaiian
Date Deposited: 23 Oct 2017 11:59
Last Modified: 23 Oct 2017 11:59
URI: http://eprints.lums.ac.ir/id/eprint/598

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