Pretreatment with Olive Leaf Extract Improves Renal and Liver Antioxidant Systems Following Renal Ischemia-Reperfusion Injury in Rats

Rasoulian, Bahram and Jafaripour, Leila and Tavafi, Majid and Rafighdoost, Houshang and Mahmodi, Mona and Rashidipour, Marzyeh and Ahmadvand, Hassan (2016) Pretreatment with Olive Leaf Extract Improves Renal and Liver Antioxidant Systems Following Renal Ischemia-Reperfusion Injury in Rats. Herbal Medicines Journal, 1 (1). pp. 37-46. ISSN 2538-2144

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Abstract

Abstract Background and Aim: The effect of Olive leaf extract (OLE) as a natural antioxidant was investigated renal and liver antioxidant enzyme activities, after renal ischemia-reperfusion (IR) in a rat model. Materials and Methods: Forty male rats divided into 5 groups: Sham (no IR), 3 "OLE+IR" groups (25mg/k 50mg/kg and 100mg/kg oral OLE once daily for 2 weeks, prior to renal IR) and "water+IR" group (oral gava of water prior to IR). In the last 4 groups, both renal pedicles clamped for 45 minutes followed by 24 hours reperfusion. Clearance of creatinine was measured. Renal and liver catalase and glutathione peroxida activities and glutathione level were assessed as well. Volume densities of the renal proximal convolut tubules were measured stereologicaly. Results: Glutathione level, catalase and glutathione peroxidase activities, clearance of creatinine and volum density of the proximal convoluted tubules decreased in "water+IR" compared to the sham group. Pretreatme with OLE in 3 different doses significantly increased renal antioxidant enzymes, creatinine clearance a volume density of the proximal convoluted tubules in comparison with "water+IR" group. Pretreatment w only one dose (25mg/kg) of OLE significantly increased liver glutathione and GPx compared to the "water+I group. Pretreatment with 2 doses (25mg/kg, 50mg/kg) increase liver catalase compared to the water+IR group Conclusions: Two weeks of oral pretreatment with different doses of OLE decreased renal injury couesd renal IR. This was associated with increased renal catalase and glutathione peroxidase activities. Some doses OLE were also associated with increased liver antioxidant system parametes. Keywords: Ischemia, Reperfusion, Olive leaf extract, Renal, Liver

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