Hejazi, Seyed-Hossein and Mirbadie, Seyed-Reza and Jafari, Rasool and Rezaiemanesh, Mohammad-Reza and Azizi, Omid and Badmasti, Farzad and Kalani, Hamed and Cheraghipour, Kourosh and Heydarian, Peyman and Hashemi, Nooshin and Izadi, Shahrokh and Jabalameli, Zahra and Mohaghegh, Mohammad-Ali (2024) Echinococcus granulosus sheep strain (G1) as the predominant genotype in definitive host (dogs) isolates in northeastern Iran. Veterinary Parasitology: Regional Studies and Reports.
Full text not available from this repository.Abstract
Echinococcus granulosus sensu lato (E. granulosus s.l.) is a zoonotic parasite, causing cystic echinococcosis in humans. In the present study, prevalence and genotypes of E. granulosus s.l. was assessed in stools collected from 244 dogs including 138 stray and 106 domestic animals using high resolution melting curve (HRM) method. Initially, to detect taeniid eggs in feces, all samples were examined using the formalin-ether techniques. Genomic DNA was extracted from the positive samples and E. granulosus s.l. was differentiated from other Taeniidae parasites using SSU-rDNA gene and E. granulosus s.l. was analyzed for genotyping using HRM based on the cox1 gene. In total, 12.7% (31/244) of the samples were positive for Taeniidae eggs. In addition, among the positive samples, 77.4% (24/31) were positive for E. granulosus s.l.. In details, 11.3% (12/106) of the domestic dogs and 8.7% (12/138) of the stray dogs were positive for E. granulosus s.l.. The results of HRM analysis showed that all E. granulosus s.l. isolates were G1 strain. Findings of the present study indicated a considerable prevalence of E. granulosus G1 among dogs in the northeast of Iran and imply a serious risk of transmitting to humans and livestock.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine R Medicine > RZ Other systems of medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 24 Jan 2024 08:25 |
| Last Modified: | 24 Jan 2024 08:25 |
| URI: | http://eprints.lums.ac.ir/id/eprint/4625 |
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