The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model

Karami,, Esmaeil and Goodarzi, Zahra and Shahtaheri, Seyed Jamaleddin and Kiani, Mehrafarin and Faridan, Mohammad and Ghazi‑Khansari, Mahmoud (2023) The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model. BMC Complementary Medicine and Therapies.

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Abstract

Background This research aimed to evaluate the protective efects of Artemisia Absinthium L. (Abs) against liver dam‑ age induced by aluminium oxide nanoparticles (Al2O3 NPs) in rats, including both structural and functional changes associated with hepatotoxicity. Methods Thirty-six rats were randomly divided into six groups (n=6). The frst group received no treatment. The sec‑ ond group was orally administered Abs at a dose of 200 mg/kg/b.w. The third and ffth groups were injected intraperi‑ toneally with γ-Al2O3 NPs and α-Al2O3 NPs, respectively, at a dose of 30 mg/kg/b.w. The fourth and sixth groups were pre-treated with oral Abs at a dose of 200 mg/kg/b.w. along with intraperitoneal injection of γ-Al2O3 NPs and α-Al2O3 NPs, respectively, at a dose of 30 mg/kg/b.w. Results Treatment with γ-Al2O3 NPs resulted in a signifcant decrease (P<0.05) in total body weight gain, relative liver weight to body weight, and liver weight in rats. However, co-administration of γ-Al2O3 NPs with Abs signifcantly increased body weight gain (P<0.05). Rats treated with Al2O3 NPs (γ and α) exhibited elevated levels of malondialde‑ hyde (MDA), inducible nitric oxide synthase (iNOS), alanine transaminase (ALT), and aspartate aminotransferase (AST). Conversely, treatment signifcantly reduced glutathione peroxidase (GPx), catalase (CAT), total superoxide dismutase (T-SOD), and total antioxidant capacity (TAC) levels compared to the control group. Furthermore, the expression of heme oxygenase-1 (HO-1) and metallothionein-1 (MT-1) mRNAs, cytochrome P450 (CYP P450) protein, and histo‑ pathological changes were signifcantly up-regulated in rats injected with Al2O3 NPs. Pre-treatment with Abs signif‑ cantly reduced MDA, AST, HO-1, and CYP P450 levels in the liver, while increasing GPx and T-SOD levels compared to rats treated with Al2O3 NPs. Conclusion The results indicate that Abs has potential protective efects against oxidative stress, up-regulation of oxidative-related genes and proteins, and histopathological alterations induced by Al2O3 NPs. Notably, γ-Al2O3 NPs exhibited greater hepatotoxicity than α-Al2O3 NPs.

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