Alpha-amylase immobilization; methods and challenges

Mafakher, Ladan and Ahmadi, Yasin and Khalili Fard, Javad and Yazdansetad, Sajjad (2023) Alpha-amylase immobilization; methods and challenges. Pharmaceutical Sciences.


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Alpha-amylase is one of the most widely used enzymes in the starch industry. However, industrial application of soluble alpha-amylase is hampered by changes in pH and temperature (adverse effects on enzyme stability) and activity loss, leading to higher costs. Immobilization of alpha-amylase is an efficient strategy to reduce the enzyme losing and subsequently reduces costs in this regard. Alpha-amylases are immobilized by adsorption, entrapment, covalent attachment, and cross-linking. A barrier in alpha-amylase immobilization is the large size of its substrate, namely amylose and amylopectin. Most of these immobilization methods decrease the affinity of the enzyme for its substrate as well as the maximum rate of reaction (Vmax). This review aims to study different aspects of alphaamylase including enzyme activity, applications, structure, starch, immobilization methods, Pharmaceutical Sciences (Indexed in ISI and Scopus) and immobilization's obstacles to improve alpha-amylase efficiency in the industry and also lowering the costs related to providing this enzyme

Item Type: Article
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: samira sepahvandy
Date Deposited: 05 Apr 2023 10:07
Last Modified: 05 Apr 2023 10:07

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