Antioxidant and cytotoxicitic effects of aqueous and alcoholic extracts of Rose Canina fruit on on the cell line U937

Falahi *, Ebrahim and Farhadi, Jafar and Abdollahpour, Foad and Ghasemi, Maryam and Kakaei, Zeynab and Karimi, Zahra (2015) Antioxidant and cytotoxicitic effects of aqueous and alcoholic extracts of Rose Canina fruit on on the cell line U937. yafte, 17 (3).

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Abstract

Background : Reactive oxygen species (ROS) can be lethal for the cell. Edible plants due to containing antioxidants reduce risk of disease and damages resulting from ROS. The aim of this study was to determine the antioxidant and cytotoxicity effects of aqueous and alcoholic fruit extracts of Rose Canina on the cell line U937. Materials and Methods: Cell line U937 (cell leukemia , human ) purchased from the Pasteur Institute were incubated for 24 hours.Oxidative stress was created by iron ( 50 mM ) or copper ( 50 mM ) and H2O2 (mM1%) for cells, and the selected antioxidants ( aqueous and alcoholic extract, 15 µmol of separate concentration levels of 25,50, and 100 mM ) were added to them (except for control samples). Glutathione levels were measured by Tietze. Lipid peroxidation was detected by MDA assay method. LDH kit was used for cytotoxicity assessments. Data were analyzed using ANOVA and Tukey’s test. Results: Mean and Sd of MDA for alcoholic extract was 0.6338 and 0.0012 µm/mg protein and for aqueous extract were 0.6389 and 0.0017 vs. for control: 0.6570 and 0.0023, respectively (p<0.05). There was no significant differentce between glutathione amounts among alcoholic and aqueous extract samples and control sample. Aqueous and alcoholic extracts 25% more than other concentrations significantly decreased the MDA. Alcoholic extract 100% had the most(62/15%) and 25% had the lowest (24/1%) toxicity. Conclusion: Alcholic and aqueous extracts of Rosa Canina fruit lead to decrease in lipid peroxidation. Their high concentrations have the most toxicity. Keywords: Rose Canina, Cell line U937, Glutathione, Antioxidant, Cytotoxicity

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