Nasiri, Saman and Mahmoudvand, Hossein and Shakibaie, Mojtaba and Mousavi, Seyyed Amir Hossein and Sepahvand, Asghar (2022) Antifungal effects of zinc nanoparticles green synthesized by Lavandula angustifolia extract, alone and combined with nystatin against Candida albicans, a major cause of oral candidiasis. Journal of HerbMed Pharmacology,.
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Abstract
Introduction: This work aimed to determine the antifungal effects of zinc nanoparticles(ZnNPs) green synthesized by Lavandula angustifolia extract, alone and along with nystatinagainst Candida albicans.Methods: ZnNPs were green synthesized with L. angustifolia extract by microwavesmethod. Antifungal effects of ZnNPs were studied by measuring the minimum inhibitoryconcentrations (MICs) and minimum fungicidal concentrations (MFCs) using the brothmicrodilution method based on the modified M27-A3 protocol on yeasts, recommendedby the Clinical and Laboratory Standards Institute (CLSI). Effects of green synthesizedZnNPs against human normal fibroblast-like Gingiva (HGF1-PI1) and human epithelial-like oral cancer (KB) cell lines were studied by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method.Results: The ZnNPs showed a spherical shape with some grains of different lengths. The bestlevels of MIC and MFC were connected to the combination of ZnNPs + nystatin with valuesof 0.204 and 0.250 μg/mL, respectively. The combination of ZnNPs with nystatin comparedto the nystatin group had a significantly better antifungal effect on C. albicans (P < 0.001). The50% cytotoxic concentrations of ZnNPs against normal (HGF1-PI1) and cancer (KB) cellswere 172.3 and 83.2 μg/mL, respectively.Conclusion: We found that ZnNPs plus nystatin had a potent antifungal effect against C.albicans. These findings indicated the cytotoxic effects of green synthesized ZnNPs oncancerous cells, whereas they were nontoxic for normal cells. Additional studies are necessaryto explain the accurate mechanism and toxicity
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RV Botanic, Thomsonian, and eclectic medicine R Medicine > RZ Other systems of medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 21 Dec 2022 05:10 |
| Last Modified: | 21 Dec 2022 05:10 |
| URI: | http://eprints.lums.ac.ir/id/eprint/4036 |
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