Isolation and Molecular identification of Lipase-Producing Bacteria from Wastewaters of a Meat Factory in Khorramabad, Iran

Khodakaramifard, Zahra and Shirazinejad*, Alireza and Mohammadi, Mohsen and Hashemi, Syed momamah bagher (2022) Isolation and Molecular identification of Lipase-Producing Bacteria from Wastewaters of a Meat Factory in Khorramabad, Iran. Yafteh, 24 (2).

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Abstract

Background: Lipases are one of the most important catalysts in the detergent, dairy, and textile industries due to their biocatalytic activity in aqueous and non-aqueous environments. The screening of different habitats to identify lipase-producing bacteria and optimizing their activity parameters facilitates the production and effective use of lipases in different industries. The present study aims to isolate and identify lipase-producing bacteria from wastewaters of a meat and investigate their characteristics. Materials and Methods: In the present study, wastewater samples from Golbahar Meat Factory in Khorramabad, Iran were screened for lipase-producing bacterial strains. The lipase-producing isolates were identified based on morphological and biochemical properties and 16S rRNA gene sequencing. The culture medium parameters such as pH, incubation time, and temperature were different for optimization. Results: Three isolates with high lipase activity were identified. These isolates were identified as Bacillus amyloliquefaciens using the nucleotide BLAST obtained for the 16S rRNA gene. the highest lipase activity for the identified isolates was recorded at 48 hours of incubation, 45 °C, and pH= 8.0. Conclusion: The alkaline lipases from Bacillus amyloliquefaciens isolates with relatively good temperature stability can be used in various industries. By identifying and isolating the lipase enzyme-producing gene from the mentioned isolates, it can be used in enzyme engineering process and the lipase activity can be changed by site-directed mutagenesis. Keywords: 16S rRNA, Bacillus amyloliquefaciens, Enzymatic activity, Lipase.

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