Ghasemian Yadegari, Javad and Khudair Khalaf, Amal and Saadatmand, Massumeh and Mahmoudvand, Hossein (2022) Antiparasitic activity of Astragalus brachycalyx subsp.brachycalyx extract against hydatid cyst protoscoleces and itseffect on induction of apoptosis: an in vitro and ex vivo study. Journal of HerbMed Pharmacology.
Full text not available from this repository.Abstract
Introduction: This study aims to evaluate in vitro and ex vivo antiparasitic activity ofAstragalus brachycalyx subsp. brachycalyx. root chloroformic extract against hydatid cystprotoscoleces and its effect on induction of apoptosis.Methods: Various concentrations of the A. brachycalyx root chloroformic extract (56.25,112.5, 225, and 450 mg/mL) were treated with hydatid cyst protoscoleces collected from theliver of infected sheep for 5-60 minutes in vitro and ex vivo. Eosin exclusion test was alsoutilized to measure the mortality of protoscoleces. Moreover, the extract effect was assessedon apoptosis induction in hydatid cyst protoscoleces by caspase-3 activity measurement.Results: The mortality rate of protoscoleces in in vitro was 100% after being exposed to 450and 225 mg/mL of A. brachycalyx extract for 20 and 30 minutes and in ex vivo for 30 and 60minutes, respectively. Following 48 h treatment of protoscoleces, A. brachycalyx chloroformicextract at the doses of 56.25, 112.5, 225, and 450 mg/mL, dose-dependently motivated thecaspase-3 enzyme ranging from 8.8% to 29.6%Conclusion: A. brachycalyx root chloroformic extract had a significant protoscolicidaleffect; however, extra surveys are required to assess its efficacy and safety as a promisingprotoscolicidal agent in clinical settings
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 30 Aug 2022 06:09 |
| Last Modified: | 30 Aug 2022 06:09 |
| URI: | http://eprints.lums.ac.ir/id/eprint/3892 |
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