Ahmadipour, Saeedeh and Varshosaz, Jaleh and Hashemibeni, Batool and Manshaei, Maziar (2022) In vivo assessment of bone repair by an injectable nanocomposite scaffold for local co-delivery of autologous platelet-rich plasma and calcitonin in rat model. Drug Dev Ind Pharm.
Full text not available from this repository.Abstract
Background: Gellan gum is obtained from the bacterium Sphingomonas elodea and is a polysaccharide with carboxylic acid functional groups. The goal of this project was to investigate the osteoinductive effect of local administration of calcitonin through an injectable scaffold of gellan gum containing salmon calcitonin loaded in silsesquioxane nanoparticles, hydroxyapatite, and platelets rich plasma.Methods: The femur of rats was defected by creating a 2 × 5 mm2 hole using an electric drill. The defect was filled with an injectable hydrogel scaffold composed of gellan gum enriched with salmon calcitonin loaded in silsesquioxane nanoparticles, hydroxyapatite, platelets rich plasma and then the radiologic images were taken. Bone densitometry and the histologic studies were carried out by Hematoxylin & Eosin test. Biochemical analysis was done to measure the serum alkaline phosphatase (ALP), calcium, calcitonin concentration.Results: Healing of the bone defects and bone densitometry in the treated group by calcitonin-loaded scaffold was significantly higher (p < 0.05) and bone formation occupied 75% of the defect that was greater than other groups. Serum ALP and calcium levels in the scaffold-loaded calcitonin group were more than the other groups (p < 0.05). The osteogenic marker genes also increased significantly (p < 0.05) with free calcitonin and the scaffold.Conclusions: Gellan gum-based scaffold loaded with calcitonin may be considered a promising local treatment to progress bone formation in repairing of skeletal injuries.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RZ Other systems of medicine |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 12 Jun 2022 03:35 |
| Last Modified: | 12 Jun 2022 03:35 |
| URI: | http://eprints.lums.ac.ir/id/eprint/3808 |
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