Detection of non-tuberculosis mycobacteria by real time pcr

Shakib, Pegah and Ahmadi, Mina and Zolfaghari, Mohammadreza (2021) Detection of non-tuberculosis mycobacteria by real time pcr. Anti-Infective Agents.

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Abstract Background: Identification of non-tuberculosis mycobacteria by culture and phenotypic description is commonly used; however, it takes 4 to 6 weeks or even a longer time for slow grow-ing species as well as for identification of some species that may be missed by biochemical charac-teristics methods. This study aimed to evaluate Real Time PCR for Detection of NTM by Amplifi-cation of Internal Transcribed Spacer (ITS) and 16S rRNA. Methods: In our investigation, using Real Time PCR and two pairs of unique primers targeted to ITS and 16S rRNA genes as well as Beta-actin as an internal control, Non tuberculosis mycobacte-ria species were detected. Results: Real time PCR was performed on the prepared dilutions. In addition, the threshold of sen-sitivity in this study was 10pg. To test the specificity, the genome of several bacteria responsible for respiratory infections was used, in which only the test response related to the non-tuberculosis mycobacterium genome and internal control was positive. Conclusion: In this research, an effective and up-to-date Real Time PCR method was used to design a diagnostic kit from all aspects. To avoid any error or mistake and to minimize the false re-sults, internal control was used. The ability to design diagnostic kits allows us to increase efficien-cy, minimize mistakes, and save a considerable amount of time and cost

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine, Health and Life Sciences > School of Medicine
Depositing User: samira sepahvandy
Date Deposited: 29 Nov 2021 07:45
Last Modified: 29 Nov 2021 07:45

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