Binding studies of the anti-retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques

Sadeghi, Marzieh and Bayat, Maryam and Cheraghi, Shekofeh and Yari, Khirollah and Heydari, Rouhollah and Dehdashtian, Sara and Shamsipur, Mojtaba (2015) Binding studies of the anti-retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques. Luminescence.

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Abstract

ABSTRACT: Interactions between efavirenz (EFZ) with calf thymus DNA (CT-DNA) were investigated in vitro under stimulated physiological conditions using multispectroscopic techniques, cyclic voltammetry viscosity measurement, and gel electrophoresis. Methylene blue and acridine orange dyes were used as spectral probes by fluorescence spectroscopy. Hypochromicity was observed in ultra-violet (UV) absorption band of EFZ. Considerable fluorescence enhancement of EFZ was observed in the presence of increasing amounts of DNA solution and the binding constants (Kf) and corresponding numbers of binding sites (n) were calculated at different temperatures. Thermodynamic parameters including enthalpy change (ΔH) and entropy change (ΔS) were calculated to be –304.78 kJmol–1 and –924.52 Jmol–1 K–1 according to the van ’t Hoff equation, which indicated that reaction is predominantly enthalpically driven. In addition, UV/vis absorption titration of DNA bases confirmed that EFZ interacted with guanine and cytosine preferentially. Gel electrophoresis of DNA with EFZ demonstrated that EFZ also has the ability to cleave supercoiled plasmidDNA. Circular dichroismstudy showed stabilization of the right-handed B formof CT-DNA. All results suggest that EFZ interacts with CT-DNA via an intercalative mode of binding. Copyright © 2015 John Wiley & Sons, Ltd.

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