A study of Salmonella Spp. contamination in egg of ducks and turkeys, consumed in Fars province

Ghorbani ranjbary*, ali and Naji, shahryar and Zarei, abdolah and Ghorbani ranjbary, nazanin (2015) A study of Salmonella Spp. contamination in egg of ducks and turkeys, consumed in Fars province. yafte, 17 (1).

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Abstract

Background : Salmonella is a short rod shape, gram-negative, non-encapsulated, aerobic and anaerobic options that cause food poisoning in humans. Raw food of animal origin, particularly meat and eggs of birds have important role in the transfer of this disease. Since duck and turkey eggs are very important in the transmission of Salmonellosis and consuming of local and non-industrial eggs of ducks and turkeys in this area is common, the present study was performed to clarify the prevalence of salmonella contamination. Materials and Methods: In the present study, 300 native duck and turkey eggs were collected and transferred to the lab. In laboratory after sampling from surface of the eggs shell were disinfected with 80% ethanol and contents of 5 duck and turkey eggs, sparately mixed in special dishes. After 24 hr incubation at 37°C with a swab in selenite - F broth were inoculated. Samples were transferred to Salmonella - Shigella agar from selenite-f environment. After incubation in 37°C, colonies suspected to Salmonella were evaluated. Suspicious colonies in the TSI and lysine decarboxylase environments were inoculated. And bacteria that had reactions related to Salmonella, were studied by PCR with specific primers for Salmonella and serotypes of Salmonella Enteritidis and Salmonella Typhimurium. Results: A total of 300 eggs of ducks and turkeys ,7 cases (2.3%) were contaminated with Salmonella, and from 7 unclean egg s shell , 1 case of turkey egg shell (0.66%) was infected with Salmonella Typhimurium. And 6 duck eggs shell (4%) were infected, that all of the serotypes were Salmonella Typhimurium. Conclusion: Regarding the present study, and other researches, it can be concluded that the Salmonella infection in the duck and turkey eggs were less than poultry eggs and it seems that vertical transmission of Salmonella in the turkeys and ducks was less than poultry. Keywords: Salmonella, Egg shell, D

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