Ahmadipour, Saeedeh and Varshosaz, Jaleh and Hashemibeni, Batool and Safaeian, Leila and Sarmadi, Akram (2020) Calcitonin-loaded octamaleimic acid–silsesquioxane nanoparticles in hydrogel scaffold support osteoinductivity in bone regeneration. Pharmaceutical Development and Technology.
Full text not available from this repository.Abstract
Novel osteoinductive scaffolds fabricated using the benefits of tissue engineering techniques accompanied by utilizing drugs can accelerate bone regeneration. The purpose of this study was to load salmon calcitonin (sCT) in octamaleimic acid-silsesquioxane (OMA-POSS) nanoparticles and enrich the hydrogel scaffold based on hydroxyapatite, Gelrite® and platelet-rich plasma (PRP) for use in bone tissue engineering. The loading efficiency, release percentage, particle size and zeta potential of the nanoparticles were evaluated. The proliferation of seeded MG-63 osteoblast cells on the designed scaffold, its cytotoxicity and osteo-conductivity were studied by alkaline phosphatase measurement and Alizarin red staining. The expression of cellular osteogenic markers such as collagen 1 (COL1A1), osteocalcin (BGLAP) and osteopontin (SPP1) was examined using reverse transcription polymerase chain reaction. The results revealed that the particle size of the nanoparticles varied between 94.2 and 199.2 nm and their negative surface charge increased after drug conjugation. The osteoblast cell proliferation and calcium granule production in the optimum formulation were significantly higher in comparison with the control group (p < 0.05). Osteogenic markers increased significantly after a specific number of days of cell culture compared to the control group (p < 0.05). The results also showed the potential of the designed scaffold in bone tissue engineering. Saeedeh AhmadipourORCID Icon,Jaleh Varshosaz ORCID Icon,Batool HashemibeniORCID Icon,Leila SafaeianORCID Icon,Maziar Manshaei &Akram Sarmadi
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 09 Jan 2021 04:52 |
| Last Modified: | 09 Jan 2021 04:52 |
| URI: | http://eprints.lums.ac.ir/id/eprint/2557 |
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