Shams, Mohammad‐Hossein and Kheirandish, Farnaz and Varzi , Ali‐Mohammad and Sohrabi , Seyyed‐Mohsen and Safari, Mojgan (2020) Molecular and Immunochemical Characterization of Pop n 2: A New Allergen of Populus nigra Pollen. Clin Exp Allergy.
Full text not available from this repository.Abstract
Background Pollen is one of the most common allergens that cause respiratory allergies worldwide. Pollen grains from poplars have been reported as important sources of pollinosis in many countries. Objective The aim of the present study was to determine the molecular and immunochemical characterization of Pop n 2, a novel allergen of Populus nigra (P. nigra) pollen extract. Methods In this study, the pollen extract of P. nigra was analyzed by SDS‐PAGE, and the allergenic profile was determined by IgE immunoblotting and specific ELISA using the sera of twenty allergic patients. The coding sequence of Pop n 2 was cloned and expressed in the Escherichia coli BL21 (DE3) using plasmid the pET‐21b (+). Finally, the expressed recombinant Pop n 2 was purified by affinity‐chromatography. Results Pop n 2 belongs to the profilin family with a molecular weight of approximately 14 kDa. Pop n 2 is the most IgE‐reactive protein (about 65%) in the P. nigra pollen extract. The cDNA sequencing results indicated an open reading frame 396 bp that encodes 131 amino acid residues. The results of ELISA and Immunoblotting assays showed that recombinant Pop n 2 could react with the IgE antibody in patients' sera, like its natural counterpart. Conclusion Our data revealed that Pop n 2 is a significant allergen in the P. nigra pollen extract. Moreover, we observed that the recombinant Pop n 2 produced by the pET‐21b (+) vector in the E. coli system acts as its natural counterpart.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > RB Pathology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Medicine |
| Depositing User: | samira sepahvandy |
| Date Deposited: | 16 Dec 2020 14:44 |
| Last Modified: | 16 Dec 2020 14:44 |
| URI: | http://eprints.lums.ac.ir/id/eprint/2493 |
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