Vasopressinattenuatesischemia–reperfusioninjuryviareduction of oxidativestressandinhibitionofmitochondrialpermeability transitionporeopeninginrathearts

Imani, AliReza and Sadr, SeyedShahabeddin and Faghihi, Mahdieh and Azizi, Yaser and Hosseini, Mir-Jamal and Mobarra, Naser and Tavakoli, Asadollah and Nazari, Afshin (2015) Vasopressinattenuatesischemia–reperfusioninjuryviareduction of oxidativestressandinhibitionofmitochondrialpermeability transitionporeopeninginrathearts. EuropeanJournalofPharmacology, 760. pp. 96-102.

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Abstract

Aim ofthisstudywastoinvestigatetheinvolvementofthemitochondrialpermeabilitytransitionpore (MPTP) andoxidativestressinthecardioprotectiveeffectofvasopressin(AVP)onischemia/reperfusion (I/R) injury.Anesthetizedmalewistarratsweresubjectedtoregional30minischemiaand120min reperfusion andrandomlydividedintoninegroups:(1)Control;salinewasadministeredintravenously before ischemia,(2)vasopressinwasadministrated10minpriortoischemia,(3,4)AtractylosideasMPTP opener,wasinjected5minpriortoreperfusionwithoutandwithvasopressin,(5,6)CyclosporineAasa MPTP closer,wasinjected5minpriortoreperfusionwithoutandwithvasopressin,(7)mitochondria wereisolatedfromcontrolgroupandCaCl2 wasaddedasMPTPopenerandswellinginducer,(8)isolated mitochondria fromControlheartswasincubatedwithCyclosporineAbeforeaddingtheCaCl2 (9) CaCl2 wasaddedtoisolatedmitochondriafromvasopressingroup.Infusionofvasopressindecreasedinfarct size (18.671.7%vs.controlgroup37.672.4%), biochemicalparameters[LDH(LactateDehydrogenase), CK-MB(CreatineKinase-MB)andMDA(Malondialdehyde)plasmalevels,PAB(Prooxidant–antioxidant balance)] comparedtocontrolgroup.Atactylosidesuppressedthecardioprotectiveeffectofvasopressin (32.571.9%vs.18.671.7%)butadministrationoftheCyclosporineAwithoutandwithvasopressin significantly reducedinfarctsizeto17.774% (Po0.001)and22.773% (Po0.01)respectively,vs. 37.672.4% incontrolgroup.Also,vasopressin,similartoCyclosporineA,ledtodecreaseinCaCl2- induced swelling.ItseemsthatvasopressinthroughantioxidanteffectandMPTPinhibitionhascreateda cardioprotectionagainstischemia/reperfusioninjuries. Keywords: Heart Ischemia/reperfusion Vasopressin Mitochondrial swelling Freeradical

Item Type: Article
Subjects: R Medicine > RS Pharmacy and materia medica
Depositing User: sobhan rezaiian
Date Deposited: 28 Sep 2016 06:45
Last Modified: 28 Sep 2016 06:45
URI: http://eprints.lums.ac.ir/id/eprint/230

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