A Stereological Study of the Toxic Effects of Cerium Oxide during Pregnancy on Kidney Tissues in Neonatal NMRI Mice

Nemati, Afsaneh and Beigi-boroujeni, Mandana and Khanipur, Zahra and Peluso, Ilaria (2020) A Stereological Study of the Toxic Effects of Cerium Oxide during Pregnancy on Kidney Tissues in Neonatal NMRI Mice. Oxidative Medicine and Cellular Longevity. (In Press)

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Abstract

Background. Both antioxidant and prooxidant activities have been previously reported for cerium oxide (CeO2). The aim of this study was to investigate the effects of CeO2 at different doses on changes in kidney tissues and markers in neonatal mice. Methods. We randomly divided 30 pregnant NMRI mice into five groups (n=6per group)—a control group and four groups treated with intraperitoneal (i.p.) administration of different doses of CeO2 (10, 25, 80, or 250mg/kg body weight (bw)) on gestation days (GD) 7 and GD14. At the end of the treatment period, we analyzed the kidney tissues and serum samples. The levels of two serum redox markers, malondialdehyde (MDA) and ferric reducing/antioxidant power (FRAP), were determined. Data were analyzed using one-way ANOVA and Tukey’s test, and a P value of <0.05 was considered significant. Results. The mean total volumes of the renal corpuscle, glomeruli, and Bowman’s capsule membranes significantly increased, and there was a significant decrease in the mean total volume of Bowman’s space in the high-dose CeO2 group compared to that in the control group. No statistically significant differences existed in the serum levels of MDA and FRAP in the treated and control groups. Conclusion. Our results suggest that high doses of CeO2 impair fetal renal development in pregnant mice, which results in kidney damage. Therefore, CeO2 administration during pregnancy could have dose-dependent adverse effects on the developing kidneys in neonates.

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