Prophylactic effects of biogenic selenium nanoparticles on acute toxoplasmosis: An in vivo study

Shakibaie, Mojtaba and Gabal, Esraa and Jahanbakhsh, Sareh and Badparva, Ebrahim (2020) Prophylactic effects of biogenic selenium nanoparticles on acute toxoplasmosis: An in vivo study. Annals of Medicine and Surgery.

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Abstract

Background: In this investigation, the in vivo efficacy and safety of biogenic selenium nanoparticles (SeNPs) are assessed against acute toxoplasmosis caused by Toxoplasma gondii (Sarcocystidae) in the mice. Methods: Male NMRI mice were orally treated with normal saline (control group) and SeNPs at the doses of 5 and 10 mg/kg once a day for 14 days. On the 15th day, the mice were infected with 104 tachyzoites of T. gondii RH strain by the intraperitoneal route. The mortality rate and parasite load were determined in the infected mice. The mRNA levels of IFN-γ, IL10, IL12, and inducible nitric oxide synthase were also examined in the infected mice by quantitative real-time PCR. Results: TherateofmortalityintheinfectedmicereceivingSeNPsatthedosesof5and10mg/kgcomparedwith the mice in the control group was 100% on the 9 and 10 days after the administration. The mean number of tachyzoites in the infected mice receiving SeNPs was significantly lower than that in the control group. No significantdifference(p > 0.05) wasfoundinthe biochemical parametersbetweenthe micetreatedwithSeNPs and the mice in the control group. The results revealed that mRNA levels significantly improved in the infected mice treated with SeNPs compared with those in the control group. Conclusion: Findings of the present investigation showed the considerable efficacy of SeNPs with no important toxicity for curing acute toxoplasmosis in the mice model. However, further studies are needed to clarify the accurate anti-Toxoplasma mechanisms of SeNPs.

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