BIOLOGICAL SOLUBILIZATION OF SOME METALS BY A NEW ACIDITHIOBACILLUS SPECIES ISOLATED FROM A MODERATE SULFUR HOT SPRING

Esmaeil Lashgarian, Hamed and Karkhane, Maryam and Marzban, Abdolrazagh (2019) BIOLOGICAL SOLUBILIZATION OF SOME METALS BY A NEW ACIDITHIOBACILLUS SPECIES ISOLATED FROM A MODERATE SULFUR HOT SPRING. J Microbiol Biotech Food Sci.

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Abstract

A chemolithotrophic bacterium was isolated from sulfur hot spring. According to phenotypic traits and 16-23S rDNA intergenic spacer region analysis, the isolate was identified and named as Acidithiobacillus sp. MR39, which was a gram-negative, rod- shape and nonmotile bacterium. The strain was able to grow in a synthetic liquid medium supplemented with the mineral ore as the source of energy. The optimum conditions were found to be within initial pH range of 2.0-2.5, at 34±1˚C and with shaking at 120 rpm. The bacterium had a remarkable potential for mineralization of 88% iron, 75% copper, 59% zinc, 59% nickel and 40% cobalt upon their growth in the liquid media. After adapting the bacterial cells to copper ions in 100 mM for 5-day incubation, biorecovery of Cu increased about 10% comparing to unadapted cells that are able to dissolve approximately 15% of total cu of mineral concentrate. Considering the finding in this study, the strain MR39 offers a great prospect for in situ extraction of metals from various ores along with other indigenous bacteria that can grow under ambient conditions.

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